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On the other hand, I think that it is derece the technical possibilities but the new samples to be analyzed (the increase in complex samples such kakım therapeutic proteins, oligonucleotides, single cell analysis, and so forth) will shape the chromatographic needs, which is now difficult to predict.
JenÅ completely shifted my interest, refocused my attention to attending lectures and I found I enthusiastically participated in his research projects. Later on, GenÅ was my supervisor during my PhD studies and we became very good friends.
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Therefore, we promote a generic method development approach which is based on the fact that proteins of the same, or similar, family (for example, mAbs or cys-linked ADCs) show very similar retention behavior. By knowing this, once you have developed a method for one mAb then it is possible to use these insights for another protein kakım it will–elute with similar mobile phase strength, show good recovery only at high temperature (75 to 90 ºC), and only elute in a sharp peak when using an ion-pairing agent and low pH. Considering these facts, at the end you only need to perform very few experiments (in a more limited range of two or three method variables) as input for further method optimization. The same input experiments güç be performed for any mAb – no need to study again the impact of method variables. Therefore, to optimize the method, retention modeling (computer simulation) can indeed be helpful.
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